Publications | DENARASE®

Viral Vectors for Gene Therapy and Vaccine Production

Selective Enhancement of REM Sleep in Male Rats through Activation of Melatonin MT1 Receptors Located in the Locus Ceruleus Norepinephrine Neurons.

Author: López-Canul, Martha et al.

Published in: The Journal of neuroscience : the official journal of the Society for Neuroscience vol. 44,29 e0914232024. (17 Jul. 2024)

Link: https://www.jneurosci.org/content/44/29/e0914232024.abstract

In vivo CAR T-cell generation in nonhuman primates using lentiviral vectors displaying a multidomain fusion ligand.

Author: Nicolai, Christopher J et al.

Published in: Blood vol. 144,9 (2024): 977-987.

Link: https://ashpublications.org/blood/article/144/9/977/516468/In-vivo-CAR-T-cell-generation-in-nonhuman-primates

Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypes.
Author: Meierrieks, Frederik et al.
Published in: Molecular therapy. Methods & clinical development vol. 31 101118. (20.0.2023)
Link: Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypes

Scaling Up of Steric Exclusion Membrane Chromatography for Lentiviral Vector Purification.
Author: Labisch et al.
Published in: Membranes 13(2): 149 (2023)
Link: Membranes | Free Full-Text | Scaling Up of Steric Exclusion Membrane Chromatography for Lentiviral Vector Purification (mdpi.com)

Efficient clinical-grade γ-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturing.
Author: Mekkaoui et al.
Published in: Molecular therapy. Methods & clinical development  28: 116-128 (2022)
Link: Efficient clinical-grade γ-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturing: Molecular Therapy - Methods & Clinical Development

Incubation Temperature and Period During Denarase Treatment and Microfiltration Affect the Yield of Recombinant Adenoviral Vectors During Downstream Processing.
Author: Sonogür et al.
Published in: Molecular Biotechnology 65(7): 1129-1139 (2023)
Link: Incubation Temperature and Period During Denarase Treatment and Microfiltration Affect the Yield of Recombinant Adenoviral Vectors During Downstream Processing | SpringerLink

Comparison of the performance of anion exchange membrane materials for adenovirus purification using laterally-fed membrane chromatography.
Author: Kawka et al.
Published in: Biochemical Engineering Journal  182: 108417 (2022)
Link: Comparison of the performance of anion exchange membrane materials for adenovirus purification using laterally-fed membrane chromatography - ScienceDirect

Steric exclusion chromatography of lentiviral vectors using hydrophilic cellulose membranes.
Author: Labisch et al.
Published in: Journal of Chromatography A 1674: 463148 (2022)
Link: Steric exclusion chromatography of lentiviral vectors using hydrophilic cellulose membranes - ScienceDirect

Integrated development of enzymatic DNA digestion and membrane chromatography processes for the purification of therapeutic adenoviruses.
Author: Kawka et al.
Published in: Separation and Purification Technology  254: 117503 (2021)
Link: Integrated development of enzymatic DNA digestion and membrane chromatography processes for the purification of therapeutic adenoviruses - ScienceDirect

A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling.
Author: Labisch et al.
Published in: Journal of Biotechnology 326: 11-20 (2021)
Link: A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling - ScienceDirect

Scalable upstream process development for the suspension-based production of lentiviral vectors for CAR T cell therapies with multiparallel & benchtop bioreactor systems & DoE methodology.
Author: Riethmüller et al.
Published in: Cell & Gene Therapy Insights 7(6): 689–700 (2021)
Link: BioInsights - Scalable upstream process development for the suspension-based production of lentiviral vectors for CAR T cell therapies with multiparallel & benchtop bioreactor systems & DoE methodology

Transfer and scale-up from 10 L BioBLU® to Allegro™ STR 50 and STR 200 Bioreactors”.
Author: Mainwaring et al.
Published in: Cell & Gene Therapy Insights 7(9): 1347–1362 (2021)
Link: BioInsights - Transfer and scale-up from 10 L BioBLU<sup>®️</sup> to Allegro™ STR 50 and STR 200 Bioreactors

Scalability comparison between 50 and 500 liter stirred tank bioreactor for production of rAAV viral vector.
Author: Sanderson et al.
Published in: Cell & Gene Therapy Insights 7(9): 1025–1033 (2021)
Link: BioInsights - Scalability comparison between 50 and 500 liter stirred tank bioreactor for production of rAAV viral vector

Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles.
Author: Hein et al.
Published in: BMC Biology 19(1): 91 (2021)
Link: Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles | BMC Biology | Full Text (biomedcentral.com)

Highly Efficient Purification of Recombinant VSV-∆ G-Spike Vaccine against SARS-CoV-2 by Flow-Through Chromatography.
Author: Lerer et al.
Published in: BioTech 10(4): 22 (2021)
Link: Highly Efficient Purification of Recombinant VSV-∆G-Spike Vaccine against SARS-CoV-2 by Flow-Through Chromatography - PMC (nih.gov)

A high cell density perfusion process for Modified Vaccinia virus Ankara production: Process integration with inline DNA digestion and cost analysis.
Author: Gränicher Gwendal, et al.
Published in: Biotechnology and Bioengineering 118(12): 4720-4734 (2021)
Link: Biotechnology & Bioengineering | Biotechnology Journal | Wiley Online Library

Production of Virus-like Particles (VLP)

Preclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells.
Author: Alpuche-Lazcano et al.
Published in: Communications medicine 3(1): 116 (2023)
Link: Preclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells | Communications Medicine (nature.com)

Development of modern immunization agent against bovine papillomavirus type 1 infection based on BPV1 L1 recombinant protein.
Author: Vrablikova et al.
Published in: Frontiers in veterinary science  10: 1116661 (2023)
Link: Frontiers | Development of modern immunization agent against bovine papillomavirus type 1 infection based on BPV1 L1 recombinant protein (frontiersin.org)

Production of antigenically stable enterovirus A71 virus-like particles in Pichia pastoris as a vaccine candidate.
Author: Kingston et al.
Published in: bioRxiv (Preprint, 2023)
Link: Production of antigenically stable enterovirus A71 virus-like particles in Pichia pastoris as a vaccine candidate | bioRxiv

VelcroVax: a "Bolt-On" Vaccine Platform for Glycoprotein Display.” 
Author: Kingston et al.
Published in: mSphere  8(1): e0056822 (2023)
Link: VelcroVax: a “Bolt-On” Vaccine Platform for Glycoprotein Display | mSphere (asm.org)

Production and Characterisation of Stabilised PV-3 Virus-like Particles Using Pichia pastoris.
Author: Sherry et al.
Published in: Viruses 14(10): 2159 (2022)
Link: Viruses | Free Full-Text | Production and Characterisation of Stabilised PV-3 Virus-like Particles Using Pichia pastoris (mdpi.com)

Protease-Independent Production of Poliovirus Virus-like Particles in Pichia pastoris: Implications for Efficient Vaccine Development and Insights into Capsid Assembly.
Author: Sherry et al.
Published in: Microbiology spectrum 11(1): e0430022 (2023)
Link: Protease-Independent Production of Poliovirus Virus-like Particles in Pichia pastoris: Implications for Efficient Vaccine Development and Insights into Capsid Assembly | Microbiology Spectrum (asm.org)

Development and preclinical evaluation of virus‐like particle vaccine against COVID‐19 infection.
Author: Yilmaz et al.
Published in: Allergy  77(1): 258-270 (2022)
Link: Development and preclinical evaluation of virus‐like particle vaccine against COVID‐19 infection - Yilmaz - 2022 - Allergy - Wiley Online Library

Separation of influenza virus‐like particles from baculovirus by polymer‐grafted anion exchanger.
Author: Reiter et al.
Published in: Journal of separation science  43(12): 2270-2278 (2020)
Link: Separation of influenza virus‐like particles from baculovirus by polymer‐grafted anion exchanger - Reiter - 2020 - Journal of Separation Science - Wiley Online Library

Production of Bacteriophages

In situ targeted base editing of bacteria in the mouse gut.
Author: Brödel, Andreas K et al.
Published in: Nature vol. 632,8026 (2024): 877-884.
Link: https://www.nature.com/articles/s41586-024-07681-w

Phage therapy potentiates second-line antibiotic treatment against pneumonic plague.
Author: Vagima et al.
Published in: Viruses 14(4): 688 (2022)
Link: Viruses | Free Full-Text | Phage Therapy Potentiates Second-Line Antibiotic Treatment against Pneumonic Plague (mdpi.com)

Biofilm Removal

Hydrolytic Enzymes as Potentiators of Antimicrobials against an Inter-Kingdom Biofilm Model.
Author: Ruiz-Sorribas et al.
Published in: Microbiology spectrum 10(1): e02589-21 (2022)
Link: Hydrolytic Enzymes as Potentiators of Antimicrobials against an Inter-Kingdom Biofilm Model | Microbiology Spectrum (asm.org)

Protein Purification

Deubiquitinating enzyme mutagenesis screens identify a USP43-dependent HIF-1 transcriptional response.

Author: Pauzaite, Tekle et al.

Published in: The EMBO journal vol. 43,17 (2024): 3677-3709.

Link: https://www.embopress.org/doi/full/10.1038/s44318-024-00166-6

UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine.
Author: Vrablikova, Alena et al.
Published in: Scientific reports vol. 13,1 19337. (7 Nov. 2023)
Link: UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine

Neuroproteomic Analysis after SARS-CoV-2 Infection Reveals Overrepresented Neurodegeneration Pathways and Disrupted Metabolic Pathways.

Author: Basak, Indranil et al.

Published in: Biomolecules vol. 13,11 1597. (30 Oct. 2023)

Link: Neuroproteomic Analysis after SARS-CoV-2 Infection Reveals Overrepresented Neurodegeneration Pathways and Disrupted Metabolic Pathways

A HIF independent oxygen-sensitive pathway for controlling cholesterol synthesis.
Author: Dickson et al.
Published in: Nature communications 14(1): 4816 (2023)
Link: A HIF independent oxygen-sensitive pathway for controlling cholesterol synthesis | Nature Communications

Diamond Quantum Sensing Revealing the Relation between Free Radicals and Huntington's Disease.
Author: Fan, S et al.
Published in: ACS central science 9(7): 1427-143 (2023)
Link: Diamond Quantum Sensing Revealing the Relation between Free Radicals and Huntington’s Disease | ACS Central Science

A histone deacetylase 3 and mitochondrial complex I axis regulates toxic formaldehyde production.
Author: Wit et al.
Published in: Science advances 9(20): eadg2235 (2023)
Link: Diamond Quantum Sensing Revealing the Relation between Free Radicals and Huntington’s Disease | ACS Central Science

Reduction in PA28αβ activation in HD mouse brain correlates to increased mHTT aggregation in cell models.
Author: Geijtenbeek et al.
Published in: PloS one 17(12): e0278130 (2022)
Link: Reduction in PA28αβ activation in HD mouse brain correlates to increased mHTT aggregation in cell models | PLOS ONE

Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding.
Author: Gajdos et al.
Published in: Nature communications 13(1): 194 (2022)
Link: Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding | Nature Communications

Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases.
Author: Dos Santos Morais et al.
Published in: Journal of Molecular Biology 434(19): 167760 (2022)
Link: Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases - ScienceDirect

A fast, efficient and easy to implement method to purify bacterial pili from Lacticasei bacillus rhamnosus GG based on multimodal chromatography.
Author: dos Santos Morais et al.
Published in: Frontiers in Microbiology 11: 609880 (2020)
Link: Frontiers | A Fast, Efficient and Easy to Implement Method to Purify Bacterial Pili From Lacticaseibacillus rhamnosus GG Based on Multimodal Chromatography (frontiersin.org)

Extracellular matrix dezellularization

3D bioprinted breast tumor-stroma models for pre-clinical drug testing.
Author: González-Callejo, Patricia et al.
Published in: Materials today. Bio vol. 23 100826. (30 Sep. 2023)
Link: 3D bioprinted breast tumor-stroma models for pre-clinical drug testing