Frequently Asked Questions about DENARASE®
Customer satisfaction is our number one priority and we look forward to help you with the application of DENARASE®. Please contact our expert team for any other questions. They will support you, discuss DENARASE® and how it can be used for your production processes.
Please find further documents about our DENARASE® products here.
What can DENARASE® be used for?
DENARASE® specifically hydrolyses the phosphodiester bonds between nucleotides, leaving smaller fragments of approximately 3-5 base pairs. The enzyme is active on all forms of nucleic acids, including single-stranded, double-stranded, linear, circular or supercoiled. DENARASE®-mediated removal of unwanted residual plasmid and host cell-derived nucleic acids can significantly improve the purification and yield of viral vectors and proteins. It is therefore widely used in research, process development and production of biologicals such as viral vectors and vaccines.
How should we add DENARASE® to our process?
There are several publications on how to use endonucleases derived from Serratia marcescens, such as DENARASE®. We recommend starting with concentrations between 10 and 60 U/mL. Several parameters such as time, concentration and temperature are highly dependent on your process conditions and should be evaluated accordingly.
Is DENARASE® active at higher salt concentrations?
The optimum salt concentration for DENARASE® is < 20 mM for monovalent ions. As shown in Figure 7 of the DENARASE® Product Information Sheet, enzyme activity decreases with increasing salt concentration (KCl & NaCl). We therefore suggest that the concentration of monovalent cations such as Na+ and K+ should be kept below 200 mM for optimal DENARASE® activity.
Is there any risk for endotoxins?
How can DENARASE® be removed from my process?
Are there different quality grades for DENARASE®?
How can we detect residual DENARASE® in our process solution?