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DENARASE® - Enzyme Characteristics

DENARASE® is a very robust enzyme that enables DNA clearance under under varying conditions. Its other enzymes. Its activity depends on various factors, such as availability of cofactors, temperature & pH. Magnesium (Mg2+) is an essential cofactor for DENARASE®. Minimal levels are required for basal and 1-2 mM Mg2+ cations for optimal DENARASE® (see Fig. 1). A large excess of MgCl2 reduces activity (see Fig. 2). The enzyme has a temperature optimum of 37°C and a pH optimum of 8.0 (see Fig. 3&4).

The effect of low and high Magnesium Chloride

Effect of low Magnesium Chloride concentrations on DENARASE® activity

Fig. 3: Effect of low magnesium chloride concentrations on DENARASE® activity

Effect of high Magnesium Chloride concentrations on DENARASE® activity

Fig. 4: Effect of high magnesium chloride concentrations on DENARASE® activity

Effect of Temperature and pH value

Effect of temperature

Fig. 1: Effect of temperature

Effect of pH value in different buffer systems-1

Fig. 2: Effect of pH value in different buffer systems

Please refer to the Validation Guide for more information on the enzyme activity.
 

Key Characteristics & Optimal Conditions

Molecular Weight (calculated):  27 kDa (per monomer)
Cofactor: Mg2+ (optimum 1-2 mM)
pH Optimum: pH 8.0-9.0 (Fig. 3)
Temperature Optimum: 37 °C (Fig. 4)
Isolectric Point (pl, calculated): 6.2

 

Product specification

In order to ensure a constant and high-quality level for DENARASE®, each batch must fulfill the in-house acceptance criteria for the parameters listed below.
 
Criteria Method Specification
Appearance visual clear, transparent solution
Activity photometric > 250 U/µL
Purity Protein purity determined by
SDS-PAGE and silver staining		 ≥ 99%
Specific Activity Activity per protein content
determined photometrically
at 280 nmwith a molar extinction
coefficient of 44,600 L x mol-1 x cm-1		 > 6 x 105 U/mg
Protease activity Protease detection assay No protease activity detectable
Endotoxin level LAL-Test acc. to Ph. Eur. 2.6.14, Method C < 0.25 EU/kU
Total microbial count TAMC/TYMC acc. to Ph. Eur. 2.6.12 Aerobic bacteria: < 5 cfu/200
µLYeast/moulds: < 5 cfu/200 µL

Unit-definition: One unit (U) will digest salmon sperm DNA to acid-soluble
oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 min at pH 8.0 at 37 °C.

Storage & Conditions

DENARASE® is stable within the specification range at a storage temperature of -20 °C for a period of at least 24 months from the date of product release. Note: It is not recommended to store the product at -70 °C or below, since freezing the product will cause loss of activity. 

Packaging Informations

DENARASE® is filled in non-pyrogenic, USP Class VI compliant vials. The product vials are shipped under qualified refrigirated conditions. The shipping temperature may differ from the recommended storage temperature without affecting product quality. All DENARASE® products will be delivered by c-LEcta in a sealed secondary packaging with tamper-evident seals. 

Get in touch with our DENARASE® expert team!

Vaishnavi Devarakonda

Vaishnavi Devarakonda
DENARASE® Sales

Need support? Contact me!
Melissa Rangel

Melissa Rangel
DENARASE® Sales

Need support? Contact me!

Sedef Ozyurek

Sedef Ozyurek
DENARASE® Sales

Need support? Contact me!