Enzyme Characteristics | DENARASE®

Cofactor
DENARASE^® uses Mg²⁺ as a cofactor and a minimum amount is needed for enzyme activity. DENARASE^® requires 1- 2 mM Mg²⁺ cations for optimal activity (see Fig. 3). A large excess of MgCl₂reduces activity (see Fig. 4).

Stability and Storage Conditions
DENARASE^® is stable within the specification range at a storage temperature of -20 °C for a period of at least 24 months from the date of product release. Note: It is not recommended to store the product at -70 °C or below, since freezing the product will cause loss of activity.

Packaging Information
DENARASE^® is filled in non-pyrogenic, USP Class VI-compliant vials. The product vials are packed in polystyrene boxes and shipped under refrigerated conditions. Shipping temperature may differ from storage temperature without affecting product quality.

Please refer to the Validation Guide for more information on the enzyme activity.

Criteria	Method	Specification
Appearance	visual	clear, transparent solution
Activity	photometric	>250 U/µL
Purity	Protein purity determined by SDS-PAGE and silver staining	≥99%
Specific Activity	Activity per protein content determined photometrically at 280 nmwith a molar extinction coefficient of 44,600 L x mol^-1 x cm^-1	>6 x 10⁵ U/mg
Protease activity	Protease detection assay	No protease activity detectable
Endotoxin level	LAL-Test acc. to Ph. Eur. 2.6.14, Method C	<0.25 EU/kU
Total microbial count	TAMC/TYMC acc. to Ph. Eur. 2.6.12	Aerobic bacteria: < 5 cfu/200 µLYeast/moulds: < 5 cfu/200 µL

DENARASE^® - Enzyme Characteristics

Effect of Temperature and pH value

Fig. 1: Effect of temperature

Fig. 2: Effect of pH value in different buffer systems

The effect of low and high Magnesium Chloride

Fig. 3: Effect of low Magnesium Chloride concentrations on DENARASE^® activity

Fig. 4: Effect of high Magnesium Chloride concentrations on DENARASE^® activity

Product specification

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DENARASE® - Enzyme Characteristics