DENARASE®

For Viral Vector Production!

  • New sizes of R&D-grade DENARASE® available

    c-LEcta has just launched additional sizes of DENARASE® for research and development use.

    Being widely applied in biopharmaceutical R&D and manufacturing, DENARASE® sizes of 1 MU and 5 MU will now also be available as R&D-grade, in addition to GMP-grade.

    Check out our video!

    Learn more

Enzymatic DNA Removal in Bioprocessing

DENARASE® is a genetically engineered endonuclease from Serratia marcescens used as a DNA eraser in purification processes of biological molecules. The enzyme cleaves all forms of DNA and RNA into smaller nucleotides of around 3-5 base pairs.

The enzyme is active in a broad range of conditions and is free of proteolytic activity. This makes the enzyme especially useful for applications with challenging DNA contents such as lysed host cells in viral vector manufacturing processes.

Because of its high efficacy, enzymatic digestion is a preferred method for reducing DNA levels well below the limits for biologicals set by the authorities.

The patented production process uses a Bacillus sp. production host. The gram-positive, endotoxin-free strain is highly productive and mitigates the risk of endotoxins in the final product. DENARASE® has a purity of ≥ 99 % and is manufactured in full compliance with GMP requirements using only raw materials of non-animal origin and without the use of antibiotics


DENARASE® is used especially in the field of:

  • Gene Therapy manufacturing
  • Cell Therapy manufacturing
  • Viral Vector purification
  • Vaccine production

DENARASE®️ - Products for use in research and development

DENARASE®️
100 kU
DENARASE®️
500 kU
DENARASE®️
1000 kU
DENARASE®️
5000 kU
DENARASE®️
Free sample
DENARASE®️
ELISA Kit

DENARASE® 100 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme cleaves all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Size: 100 kU
Purity: ≥ 99%​
Activity: > 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced in conformity with ISO 9001 standard
  • Manufacturing free of animal derived raw materials and antibiotics
  • Depending on your application 10 – 60 U/ml is needed ​
  • net price excluding VAT and shipment costs

DENARASE®️ 500 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme cleaves all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Size: 500 kU
Purity: ≥ 99%​
Activity:
> 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced in conformity with ISO 9001 standard
  • Manufacturing free of animal derived raw materials and antibiotics
  • Depending on your application 10 – 60 U/ml is needed ​
  • net price excluding VAT and shipment costs

DENARASE® 1000 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme cleaves all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Size: 1000 kU
Purity: ≥ 99%​
Activity: > 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced in conformity with ISO 9001 standard
  • Manufacturing free of animal derived raw materials and antibiotics
  • Depending on your application 10 – 60 U/ml is needed ​
  • net price excluding VAT and shipment costs

In order to avoid mix-ups, packaging units of the same size are indicated differently in the product name of the two quality grades: 1000/5000 kU for R&D-grade DENARASE® and 1/5 MU for GMP-grade DENARASE®

DENARASE®️ 5000 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme cleaves all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Size: 5000 kU
Purity: ≥ 99%​
Activity:
> 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced in conformity with ISO 9001 standard
  • Manufacturing free of animal derived raw materials and antibiotics
  • Depending on your application 10 – 60 U/ml is needed ​
  • net price excluding VAT

In order to avoid mix-ups, packaging units of the same size are indicated differently in the product name of the two quality grades: 1000/5000 kU for R&D-grade DENARASE® and 1/5 MU for GMP-grade DENARASE®

Free sample DENARASE 25 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme cleaves all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Size: 25 kU
Purity: ≥ 99%​
Activity:
> 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Complies with ISO 9001 conditions
  • Manufacturing free of animal derived raw materials and antibiotics
  • Depending on your application 10 – 60 U/ml is needed ​
  • sample is shipped DAP (delivered at place)

ELISA Kit for Benzonase* and DENARASE®️

For the quantitative analysis of residual serratia marcescens endonucleases in process samples.

Save 20% on your first ELISA Kit with code ELISAKit_20

  • The kit consists of a pre-coated micro test plate and all ready to use reagents. ​
  • The capacity of 1 kit is 480 measurements ​
  • Estimated Delivery time: 1-2 weeks (depending on region)​
  • net price excluding VAT and shipment costs

*Benzonase Nuclease is a registered trademark of Merck KGaA

DENARASE®️ - GMP products for biopharmaceutical manufacturing

DENARASE®
1 MU, GMP
DENARASE®
5 MU, GMP
DENARASE®
ELISA Kit

DENARASE®️ 1 MU, GMP

For GMP manufacturing of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Size: 1 MU, GMP
Purity: ≥ 99%​
Activity: > 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced under EU GMP conditions
  • Manufacturing free of animal derived raw materials and antibiotics
  • Dedicated regulatory support for US-market approvals of pharmaceutical products via own US FDA Drug Master File
  • Net price excluding VAT and shipment costs

In order to avoid mix-ups, packaging units of the same size are indicated differently in the product name of the two quality grades: 1000/5000 kU for R&D-grade DENARASE® and 1/5 MU for GMP-grade DENARASE®

DENARASE®️ 5 MU, GMP

For GMP manufacturing of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Size: 5 MU, GMP
Purity: ≥ 99%​
Activity: > 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced under EU GMP conditions
  • Manufacturing free of animal derived raw materials and antibiotics
  • Dedicated regulatory support for US-market approvals of pharmaceutical products via own US FDA Drug Master File
  • Net price excluding VAT and shipment costs

In order to avoid mix-ups, packaging units of the same size are indicated differently in the product name of the two quality grades: 1000/5000 kU for R&D-grade DENARASE® and 1/5 MU for GMP-grade DENARASE®

ELISA Kit for Benzonase* and DENARASE®️

For the quantitative analysis of residual serratia marcescens endonucleases in process samples.

Save 20% on your first ELISA Kit with code ELISAKit_20

  • The kit consists of a pre-coated micro test plate and all ready to use reagents. ​
  • The capacity of 1 kit is 480 measurements ​
  • Estimated Delivery time: 1-2 weeks (depending on region)​
  • net price excluding VAT and shipment costs

*Benzonase Nuclease is a registered trademark of Merck KGaA

Not quite ready to buy yet?

  • Get your free sample DENARASE!

    New customers from the pharmaceutical industry can request a free 25 kU sample for first lab trials.

  • ELISA-Kit for analytical support

    For the quantitative analysis of endonucleases from Serratia marcescens a DENARASE® ELISA detection kit is available.

    Learn more

Get in touch with our DENARASE expert team!

Vaishnavi Devarakonda
Vaishnavi Devarakonda DENARASE Sales +49-341 3552 140
Melissa Rangel
Melissa Rangel DENARASE Sales +49-341 3552 140
José Vázquez
José Vázquez Key-Account-Manager DENARASE +49-341 3552 140

Speak to a member of our expert team and learn more about our DENARASE products!

Qualified and used
by big pharma companies

  • „We have used DENARASE in our manufacturing production. It is good according to our test. We plan to use it in next valuable schedule.”

    US-Children Hospital, USA,
    Clinical Vector Core, Lead Scientist
  • „I tested the DENARASE yesterday and it was equally good as our standard in my setting. I will continue using it in variety of conditions that I normally use and I will most certainly be purchasing my future enzymes from you.”

    US University, USA,
    Scientist
  • „We are going to use DENARASE exclusively and no longer our usual nuclease. The product is excellent in performance and price.”

    US biopharmaceutical manufacturer, USA,
    Lab Manager Vector Production